Belongs to the aggrecan/versican proteoglycan family. It is encoded by the VCAN gene. This gene is a member of the aggrecan/versican proteoglycan family. 2282-2405: 2.97e-97: C-type lectin-like domain (CTLD) of the type found in chondroitin sulfate proteoglycan core proteins; CLECT_CSPGs: C-type lectin-like domain (CTLD) of the typ Contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Aggrecan, the best understood of the lecticans, is fundamental for cartilage function and skeletal development. Aggrecan - Wikipedia Fibulin-1 binds to a number of extracellular matrix constituents including fibronectin, nidogen-1, and the proteoglycan, versican. Description. Storage & Handling. Our webinar attendees requested a webinar on Lymphatics, so please join us on August 2 at 1:00pm EDT for Dr. Dellinger's presentation, "Lymphatic Vessels and Vanishing Bones: Animal Models of Lymphatic Anomalies with Bone Involvement."Dr. Contains 1 C-type lectin domain. Anat Embryol. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Mouse anti Human Aggrecan antibody, clone 7D4 recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). The predominant proteoglycan present in cartilage is the large chondroitin sulfate proteoglycan ‘aggrecan’. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteases regulate proteoglycan content. This antibody reacts with Human, Rat. Tested in IHC applications. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Anti-Aggrecan ACAN Antibody. A gene on chromosome 5q14.3 that encodes an aggrecan/versican proteoglycan, which is a large chondroitin sulphate and major component of the extracellular matrix involved in cell adhesion, proliferation, migration and angiogenesis, and which plays a central role in tissue morphogenesis and maintenance. Abstract The proteoglycan aggregate is the major structural component of the extracellular matrix of the cartilage, composed of aggrecan, hyaluronan (HA) and link protein (LP). Article Download PDF Google Scholar Store at -20°C for one year. Large aggregates of aggrecan and versican were detected in ascending aortas from patients with TAAD and mice with Marfan syndrome, 13,14 particularly in dissected and ruptured aortas. Form. It binds avidly to hyaluronic acid via an N-terminal globular region. Background Information Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. Belongs to the aggrecan/versican proteoglycan family. cDNA clones encoding proteins related to the aggrecan/versican family of proteoglycan core proteins have been isolated with antisera against rat brain synaptic junctions. Between the G1 and G2 domains there is an interglobulin region (IGD). It is a major component of extracellular matrix of cartilaginous tissues. Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the lectican ( chondroitin sulfate proteoglycan ) family. Cartilage contains up to 10% proteoglycan consisting of mainly the large aggregating chondroitin sulfate proteoglycan aggrecan. Aggrecan is a member of the hyaluronan (HA)-binding proteoglycan family (which also includes brevican, neurocan, and versican) and associates noncovalently with HA and the 45kDa link glycoprotein to form high molecular weight aggregates (>200MDa). Contains 1 C-type lectin domain. Yamada et al. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. Contains 1 Ig-like V-type (immunoglobulin-like) domain. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. The extracellular matrix (ECM) proteoglycan, versican increases along with other ECM versican binding molecules such as hyaluronan, tumor necrosis factor stimulated gene-6 (TSG-6), and inter alpha trypsin inhibitor (IαI) during inflammation in a number of different diseases such as cardiovascular and lung disease, autoimmune diseases, and several different cancers. See all ACAN products. Boster Bio Anti-Aggrecan ACAN Antibody catalog # A04655-1. The lecticans are a family of chondroitin sulfate proteoglycans including aggrecan, versican, neurocan, and brevican. This antibody reacts with Human, Rat. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Boster Bio Anti-Aggrecan core protein ACAN Antibody catalog # A04655. Decreased ADAMTS activity results in proteoglycan accumulation, which has been associated with lipid retention and atherosclerosis, as well as elastin breaks and aortic aneurysm formation. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. 18 The other hyalectans, namely versican, brevican, and neurocan, have fewer CS chains than aggrecan, with versican having the most, … 100ul. Contains 2 Link domains. Following its secretion, aggrecan self-assembles into a supramolecular structure with as many as 50 monomers bound to a filament of hyaluronan. Contains 1 C-type lectin domain. the aggrecan/versican family. May play a regulatory role in the matrix assembly of the cartilage. One of the cDNA groups has been fully sequenced and shown to encode a novel proteoglycan core protein of the aggrecan/versican family. Molecular Weight: Various Aggrecan can contain upwards of 100 chondroitin sulfate chains and can also contain keratan sulfate … Aggrecan is a major component of cartilage extracellular matrix. This gene is a member of the aggrecan/versican proteoglycan family. Function i This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Thus far these proteoglycans include versican, neurocan, and brevican. Versican (proteoglycan-mesenchyme) is a proteoglycan related to aggrecan expressed in smooth muscle. Versican binds to type I, II, III, and V collagens and tenascin. See all ACAN products. Sample Preparation For Western Blotting. Molecular pathology. [3] This gene is a member of the lectican (chondroitin sulfate proteoglycan) family.The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Contains 1 Sushi (CCP/SCR) domain. proteoglycans,encompassingaggrecan,versican,neuro- adult brain, lecticans are thought to interact with can and brevican. Yamagata M, Shinomura T, Kimata K. Tissue variation of two large chondroitin sulfate proteoglycans (PG-M/versican and PG-H/aggrecan) in chick embryos. Contains 1 Ig-like V-type (immunoglobulin-like) domain. 28. In car-tilage, these aggregates form … 3 isoforms have been identified. Multiple cells types release extracellular proteases and ECM molecules that assemble in diverse forms of ECM with multiple functional roles (upper panel). 1A). These proteoglycans are character- hyaluronanandtenascin-Rtoformaternarycomplex. (1994) described the molecular cloning of a novel bovine brain proteoglycan of the aggrecan/versican/neurocan family. Here, we defined the aortic proteoglycanome using mass spectrometry, and based on the findings, investigated the large aggregating proteoglycans aggrecan and versican in human ascending TAAD and a mouse model of severe Marfan syndrome. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Aggrecan: | |Aggrecan|, also known as |cartilage-specific proteoglycan core protein| (CSPCP) or |chon... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, and the most definitive collection ever assembled. The aggregates provide cartilage with unique gel-like property and resistance to deformation through water absorption. Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. 1993;187:433-44 29. The aggrecan family includes other important members such as versican, also named PG-M, neurocan, brevican and the cell surface HA receptor CD44. PG-M and PG-H, chick large chondroitin sulfate proteoglycans corresponding to versican (fibroblasttype proteoglycan) and aggrecan (cartilage-characteristic proteoglycan), respectively, which are found in mammals, have been characterized in various tissues of chick embryos. SKU/Catalog Number. The aggregating proteoglycan network formed by aggrecan, link protein, and hyaluronan provides biomechanical properties that give the tissue its ability to withstand and distribute load. Tested in WB applications. Aggrecan is a high molecular weight (1x10 < M < 3x10 ) proteoglycan. Form. A04655. Aggrecan is composed of three major domains: G1, G2, and G3. ), Biology of Proteoglycans, Academic Press, Orlando, FL (1987), p.1. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Contains 1 C-type lectin domain. In … The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. A major function of this protein is to resist compression in cartilage. Size. Description: Homo sapiens versican (VCAN), transcript variant 4, mRNA. Belongs to the aggrecan/versican proteoglycan family. Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to the aggrecan/versican proteoglycan family and is a major component of the extracellular matrix of cartilagenous tissues. Size. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. The aggrecanase and proteoglycanase clades (ADAMTS1, 4, 5, 8, 15, and ADAMTS9 and 20) can cleave hyaluronan-binding chondroitin sulfate proteoglycan (CSPG) extracellular proteins, including aggrecan, versican, brevican and neurocan []. Neurocan is a developmentally regulated CSPG that shares functional domains with the cartilage CSPG aggrecan and the fibroblast CSPG versican . Aggrecan itself is the most well characterized member of aggrecan family and is the major proteoglycan in cartilage. Brevican is a member of the aggrecan/versican family of proteoglycans, containing a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Protocols & Troubleshooting IHC Protocol. No information is available about expression or localization of these ADAMTSs in teeth. It is a member of the aggrecan/versican proteoglycan family. The extent of glycosaminoglycan side chain substitution in aggrecan and resulting fixed charge density attracts counter-ions and water, resulting in swelling pressure that is crucial for the biomechanical … Dellinger is an Assistant Professor at … A major function of aggrecan … ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. These hyaluronan-binding proteoglycans are the main components responsible for vascular viscosity. The C-terminal globular domains of lecticans are structurally related to selectins, consisting of a C-type lectin domain flanked by epidermal growth factor and complement regulatory protein domains. 3 isoforms have been identified. Versican (versatile proteoglycan; also known as PG-M) is a 500-600kD, secreted member of the Aggrecan/Versican proteoglycan family. Proteoglycan Family The structure and properties of aggrecan are similar to those of other large chondroitin sulfate (CS) pro-teoglycans collectively known as hyalectans, lecti-cans, or aggregating proteoglycans, which bind to the GAG hyaluronan (HA) to form large aggregates.18 The other hyalectans, namely versican, brevican, and The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. Contains 1 Sushi (CCP/SCR) domain. Provided below are ELISA kits targeting aggrecan, a human protein encoded by ACAN. This gene is a member of the aggrecan/versican proteoglycan family. Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. In the present study we immunolocalized versican and aggrecan Cspgs during chick elbow joint morphogenesis between HH st25-41 of development. This is reported to be a secreted protein. Anti-Aggrecan core protein ACAN Antibody. Aggrecan, link protein and TN-R were identified to be essential for the neuroprotective properties of PN, whereas the contribution of brevican was negligible. Contains 1 Sushi (CCP/SCR) domain. Antigen Description This gene is a member of the aggrecan/versican proteoglycan family. The first of these is the lectican family, including aggrecan, versican, and the cartilage link protein. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Wight, R.P. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. A04655-1. 100ul. Yamada et al. This extremely highly charged proteoglycan was named for its ability to form large aggregates in the cartilage tissue. Brevican has the smallest core protein among this family and is one of the most abundant chondroitin sulfate proteoglycans … Store at -20°C for one year. It exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate glycosaminoglycan (GAG) chains are attached to an extended protein core. T.N. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Western Blotting Handbook. aggrecan(ACAN) Homo sapiens This gene is a member of the aggrecan/versican proteoglycan family. 1A). Chondroitin sulfate proteoglycan; binds to hyaluronic acid; Belongs to the aggrecan/versican proteoglycan family : 0.968: HSPG2: Basement membrane-specific heparan sulfate proteoglycan core protein; Integral component of basement membranes. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. Two sets of overlapping cDNAs have been characterized that differ in their 3′-terminal regions. For instance, the aggrecan gene family consists of four distinct proteoglycans—aggrecan, versican, neurocan, and brevican. Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan … For aggrecan and cartilage link protein, both Link modules in the type C domain are known to be involved in the interaction with HA (12, 13); therefore, it seems likely that two Link modules (and in some cases the Ig domain) are necessary Contains 2 EGF-like domains. Contains 1 C-type lectin domain. (from RefSeq NM_001164098) RefSeq Summary (NM_001164098): This gene is a member of the aggrecan/versican proteoglycan family. A major function of this protein is to resist compression in cartilage. Mecham (Eds. Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. (1994) described the molecular cloning of a novel bovine brain proteoglycan of the aggrecan/versican/neurocan family. This family, also known as hyalectans, includes aggrecan, versican, neurocan, and brevican. IF/ICC Protocol. SEQUENCE SIMILARITIES: Belongs to the aggrecan/versican proteoglycan family. “Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that in humans is encoded by the ACAN gene. Storage & Handling. Description. ACAN proteoglycan mainly consists of chondroitin sulfate, however it also contains keratan sulfate chains and N/O-linked oligosaccharides. Members of the family of large chondroitin sulfate proteoglycans (CSPGs), such as versican and aggrecan, are involved in early heart development, and in … Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. A major function of aggrecan is to resist compression in cartilage. This is reported to be a secreted protein. CSPGs belonging to the hyalectin family include aggrecan and versican. Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. Liquid. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Yamagata M, Kimata K. Repression of a malignant cell-substratum adhesion phenotype by inhibiting the production of the anti-adhesive proteoglycan, PG-M/versican. Belongs to the aggrecan/versican proteoglycan family. Liquid. Versican is a large chondroitin sulfate proteoglycan and belongs to the family of lecticans (Aspberg et al., 1997; Yamaguchi, 2000).It was initially identified in cultured fibroblasts (Zimmermann and Ruoslahti, 1989) and its chicken homologue (called PG-M) was isolated from developing limb buds (Shinomura et al., 1993).Versican possesses two globular domains, the N … Contains 2 Link domains. Proteoglycan accumulation is a hallmark of medial degeneration in thoracic aortic aneurysm and dissection (TAAD). The core protein of versican is encoded by the VCAN gene. Decorin mRNA was the most highly-expressed of the proteoglycan mRNA, while versican mRNA expression was higher (3.8-fold) than that of aggrecan. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Versican is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Cartilage contains up to 10% proteoglycan consisting of mainly the large aggregating chondroitin sulfate proteoglycan aggrecan. The aggrecan family includes other important members such as versican, also named PG-M, neurocan, brevican and the cell surface HA receptor CD44 8, 9, 10, 11. Contains 2 EGF-like domains. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Contains 2 EGF-like domains. Neurocan is a developmentally regulated CSPG that shares functional domains with the cartilage CSPG aggrecan and the fibroblast CSPG versican . VCAN. Like all hyalectins, aggrecan and versican are large proteoglycans with numerous CS substitution sites (Fig. INTRODUCTION. In contrast, CSPG members of the decorin family (decorin and biglycan) are smaller and have fewer CS substitution sites (Fig. Contains 2 EGF-like domains. Belongs to the aggrecan/versican proteoglycan family. Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). Contains 1 Sushi (CCP/SCR) domain. The aggrecan family … SKU/Catalog Number. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. This gene is a member of the aggrecan/versican proteoglycan family. Contains 1 Sushi (CCP/SCR) domain. Contains 4 Link domains. The lane on the left was treated with blocking peptide. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. proteoglycan (CSPG) family, aggrecan, versican, neurocan, and brevican (2). The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. This proteoglycan, named brevican, carries chondroitin sulfate chains, and, like other members of the family, contains a hyaluronic acid-binding domain in its N-terminal region, an epidermal growth factor-like repeat, a lectin-like and a … Technical Notes: Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry (1, 2). Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Aggrecan (cartilage-specific proteoglycan core protein, chondroitin sulphate proteoglycan 1, aggregating chondroitin sulphate proteoglycan) is the product of the Acan gene and a member of the aggrecan/versican/proteoglycan family of extracellular matrix proteins. aggrecan: ( ag're-kan ), Candidate gene for otosclerosis located at 15q25 to q26. Contains 2 Link domains. This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. This gene is a member of the aggrecan/versican proteoglycan family. Aggrecan serves a direct, primary role pro- A much as 10% of the dry weight of some cartilages is aggrecan. The large chondroitin sulfate proteoglycan, aggrecan, is the predominant proteoglycan present in cartilage. Versican belongs to the family of the large aggregating chondroitin sulfate proteoglycans located primarily within the extracellular matrix (ECM). Mutations in this gene may be involved … This gene is a member of the aggrecan/versican proteoglycan family. VCAN versican [ (human)] Summary. This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. The 19 human ADAMTS proteins can be assembled into eight ‘clades’ on the basis of their domain organization and their known functions. It binds avidly to hyaluronic acid via an N-terminal globular region. aggrecan: Background: This gene is a member of the aggrecan/versican proteoglycan family. Members of the ADAMTS family degrade versican and aggrecan. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and One such family includes several proteoglycans that have been grouped with aggrecan, the large aggregating chondroitin sulfate proteoglycan of cartilage, based on a high number of sequence similarities within the N- and C-terminal domains. metalloproteinases), hyaluronan-binding proteoglycans, such as versican and aggrecan, are mainly regulated by members of the ADAMTS family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. This protein is 2530 amino acids long, with a mass of 261.3 kDa, and is a member of the Aggrecan/versican proteoglycan family. Entrez Gene Summary. It is a major component of extracellular matrix of cartilaginous tissues. Thus, members of the Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Mutations in this gene may be involved … The structure and properties of aggrecan are similar to those of other large chondroitin sulfate (CS) proteoglycans collectively known as hyalectans, lecticans, or aggregating proteoglycans, which bind to the GAG hyaluronan (HA) to form large aggregates. Contains 2 Link domains. A disintegrin and metalloprotease with thrombospondin type 1 motifs (ADAMTS) is a family of extracellular proteases and implicated in cleaving proteoglycans, such as aggrecan, versican and brevican. Provided below are ELISA kits targeting aggrecan, a human protein encoded by ACAN. Large aggregates of aggrecan and versican were detected in ascending aortas from patients with TAAD and mice with Marfan syndrome, 13,14 particularly in dissected and ruptured aortas. WB Images(2) Western blot analysis of extracts from 3T3, using Aggrecan Antibody. This protein is 2530 amino acids long, with a mass of 261.3 kDa, and is a member of the Aggrecan/versican proteoglycan family.
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